🔹 What is Probiotic Toxicity and Inflammation Assay on Organ Chip?

The Probiotic Toxicity and Inflammation Assay on Organ Chip is an advanced in vitro platform that uses microfluidic organ-on-a-chip technology to evaluate the safety and immunomodulatory effects of probiotic strains.

By mimicking physiological tissue microenvironments, this assay allows researchers to study host responses, inflammatory signaling, and potential cytotoxicity of probiotics in a controlled and human-relevant context.

🔹 Key Features

• Organ-on-a-Chip Platform:

  • Gut, liver, or multi-organ chips simulate tissue architecture, fluid flow, and cell-cell interactions.

• Probiotic Evaluation:

  • Test live probiotic strains or microbial consortia for safety and functional effects.

• Inflammatory Readouts:

  • Cytokine profiling, immune cell activation, barrier integrity (TEER), and gene expression analysis.

• Toxicity Assessment:

  • Evaluate cytotoxicity, epithelial damage, and metabolic perturbations.

• High-Throughput & Physiologically Relevant:

  • Allows multiple strains or conditions to be tested under dynamic flow conditions.

🔹 Applications

• Safety Evaluation:

  • Assess potential toxic effects of probiotics before preclinical or clinical use.

• Mechanistic Studies:

  • Investigate probiotic-induced modulation of inflammation, immune responses, or epithelial function.

• Therapeutic Screening:

  • Select probiotic strains with optimal safety and immunomodulatory profiles.

• Microbiome-Host Interaction Research:

  • Study how probiotics influence gut-liver or gut-immune axis in a controlled organ-like environment.

✅ In summary:
The Probiotic Toxicity and Inflammation Assay on Organ Chip provides a human-relevant, physiologically accurate platform to evaluate safety and immunomodulatory effects of probiotics, supporting drug discovery, functional screening, and translational microbiome research.